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Subculture on potato dextrose agar as a complement to the broth microdilution assay for Malassezia pachydermatis

2008; Elsevier BV; Volume: 75; Issue: 2 Linguagem: Inglês

10.1016/j.mimet.2008.05.022

1872-8359

Marilena R. Prado, Érika Helena Salles de Brito, Raimunda Sâmia Nogueira Brilhante, Rossana de Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha,

Fungal Biology and Applications

The main aim of this study was to verify the efficacy of subculture on potato dextrose agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n = 50) had shown MIC values of < 0.03, < 0.03, 2.0, 4.0 and 4.0 μg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.