Suppression of Gene Amplification in Human Cell Hybrids
1992; American Association for the Advancement of Science; Volume: 255; Issue: 5050 Linguagem: Inglês
10.1126/science.1542791
ISSN1095-9203
AutoresThea D. Tlsty, Alicia White, Janet Sanchez,
Tópico(s)Genomic variations and chromosomal abnormalities
ResumoGene amplification, one example of genetic instability, is of prognostic and clinical importance in neoplasia. In tumorigenic cells, gene amplification occurs at a very high frequency, whereas in normal diploid fibroblasts the event is undetectable by the clonogenic assay. To investigate genetic control of gene amplification, amplification frequency was measured in hybrids of tumorigenic cells and normal diploid cells. The ability to amplify an endogenous gene behaved as a recessive genetic trait, and control of gene amplification potential segregated independently of tumorigenicity and immortality.
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