Microbiological High Throughput Screening: An Opportunity for the Lead Discovery Process
2000; Elsevier BV; Volume: 5; Issue: 1 Linguagem: Inglês
10.1177/108705710000500105
ISSN2472-5560
AutoresMarie-Hélène Beydon, Alain Fournier, Lionel Drugeault, Jérôme Becquart,
Tópico(s)Genomics and Phylogenetic Studies
ResumoMicrobial HTS has been implemented at Rhône-Poulenc Rorer through the development of a dedicated robotic platform. This robot (Turbo) has been designed with the aim of fully integrating microbial HTS into the lead discovery processes. Innovative solutions have been found to reach high throughput as well as flexibility. This opens up new prospects for solid-phase microbial screening, taking advantage of the easy implementation and the very low costs of such screens. The different types of microbial screens done in our laboratory, as well as the throughputs and outputs obtained, are described. Some of the specific aspects of microbial HTS, as compared to biochemical and cell-based assays, are also discussed.
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