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Peptide photodissociation at 157 nm in a linear ion trap mass spectrometer

2005; Wiley; Volume: 19; Issue: 12 Linguagem: Inglês

10.1002/rcm.1969

1097-0231

Taeyoung Kim, Matthew S. Thompson, James P. Reilly,

Analytical Chemistry and Chromatography

Abstract The photodissociation by 157 nm light of singly‐ and doubly‐charged peptide ions containing C‐ or N‐terminal arginine residues was studied in a linear ion trap mass spectrometer. Singly‐charged peptides yielded primarily x ‐ and a ‐type ions, depending on the location of the arginine residue, along with some related side‐chain fragments. These results are consistent with our previous work using a tandem time‐of‐flight (TOF) instrument with a vacuum matrix‐assisted laser desorption/ionization (MALDI) source. Thus, the different internal energies of precursor ions in the two experiments seem to have little effect on their photofragmentation. For doubly‐charged peptides, the dominant fragments observed in both photodissociation and collisionally induced dissociation (CID) experiments are b ‐ and y ‐type ions. Preliminary experiments demonstrating fragmentation of multiply‐charged ubiquitin ions by 157 nm photodissociation are also presented. Copyright © 2005 John Wiley & Sons, Ltd.