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Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats

1990; Elsevier BV; Volume: 1045; Issue: 3 Linguagem: Inglês

10.1016/0005-2760(90)90126-i

1879-145X

Pam Fredman, Jan‐Eric Månsson, Sandra H. Bigner, Carol J. Wikstrand, Darell D. Bigner, Lars Svennerholm,

Proteoglycans and glycosaminoglycans research

This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.