Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Effect of coconut water on growth of olive embryos cultured in vitro

2013; UNIVERSIDADE FEDERAL DE SANTA MARIA; Volume: 43; Issue: 2 Linguagem: Inglês

10.1590/s0103-84782013000200016

1678-4596

Rafaeli Aparecida Vieira de Souza, Francyane Tavares Braga, Tesfahun Alemu Setotaw, João Vieira Neto, Patrícia Helena de Azevedo, V. H. de Azevedo, Geraldo Magela de Almeida Cançado,

Plant Genetic and Mutation Studies

The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means of measured parameters. However, the study showed the importance of determining the appropriate dose of coconut water for each genotype under consideration as shown by significant genotype x dose of coconut water interaction effect.