INTRANUCLEAR SYNTHESIS OF POLYGLUCOSE PARTICLES UNDER HISTOCHEMICAL CONDITIONS IN THE NORMAL RAT
1975; Japan Society of Histochemistry and Cytochemistry; Volume: 8; Issue: 4 Linguagem: Inglês
10.1267/ahc.8.247
ISSN1347-5800
AutoresHaruhiko Miyayama, Michio Okabe, KOJI SHIGENAGA, Tadao Takeuchi,
Tópico(s)Metabolism, Diabetes, and Cancer
ResumoPolyglucose particles artificially synthesized under histochemical conditions from glucose 1-phosphate by phosphorylase and branching glycosyltransferase in the nuclei of skeletal muscle fibers, satellite cells and endothelial cells of the blood capillary of the normal rat were studied at the electronmicroscopic level.Newly formed polyglucose particles appeared in the karyolymph and interchromatin area of nuclei as a large macromolecular structure of spheroidal branching bodies. The size of intranuclear polyglucose particles ranging from 400 Å to 1600 Å in diameter was slightly larger than the cytoplasmic particles.There was no evidence that newly formed particles in the nuclei had a relation to the chromatin or the nuclear membrane.
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