Determination of plasma levels of exemestane (FCE 24304), a new irreversible aromatase inhibitor, using liquid chromatography/thermospray mass spectrometry
1995; Wiley; Volume: 30; Issue: 5 Linguagem: Inglês
10.1002/jms.1190300506
ISSN1096-9888
AutoresCecilia Allievi, P. Zugnoni, M. Strolin Benedetti, P. Dostert,
Tópico(s)Estrogen and related hormone effects
ResumoAbstract A high performance liquid chromatographic mass spectrometric method with thermospray ionization for the determination of concentrations of exemestane (6‐methylenandrosta‐1,4‐diene‐3,17‐dione, FCE 24304) in human plasma has been developed. The sensitivity of the method allowed the determination of exemestane concentrations as low as 1 ng ml −1 . A suitable internal standard was used for quantification. The intra‐day precision (relative standard deviation, R.S.D.) ranged from 16.3% near to the lower limit of quantification to 0.3% close to the upper end of the calibration graph. The mean accuracy of the method was found to be 97.8% (R.S.D.=7%). Unlike a previously developed gas chromatographic/mass spectrometic method, the new method proved to be suitable for the determination of unchanged drug plasma levels in exemestane‐treated subjects.
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