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Pentose shunt enzyme activities in non‐inflamed attached gingiva of dogs

1973; Wiley; Volume: 8; Issue: 1 Linguagem: Inglês

10.1111/j.1600-0765.1973.tb00736.x

1600-0765

J. I. Bergquist, Klaus Nuki,

Proteoglycans and glycosaminoglycans research

Ultramicrochemical methods were used to assay glucose‐6‐phosphate dehydrogenase (G‐6‐PDH) and 6‐phosphogluconale dehydrogenase (6‐PGDH) activities in non‐inflamed attached gingiva of dogs, these enzymes being an integral part of the pentose shunt. Precautions were taken to assure the clinical non‐inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 12 to 240 nanograms (10 ‐9 grams). Quantitative results for various strata of epithelium, connective tissue and whole epithelium were calculated. The mean combined G‐6‐PDH and 6‐PGDH activities in micromoles of substrate converted., per gram of dry tissue per minute at 37° C. were: surface layer, 17.7; granular layer, 56.4; spinous layer, 58.1; basal layer, 49.5; connective tissue, 15.8; and whole epithelium, 46.6. The mean 6‐PGDH activities in micromoles of substrate converted per gram of dry tissue per minute at 37° C. were: surface layer, 6.8; granular layer, 22.1; spinous layer, 17.5; basal layer, 20.8; connective tissue, 4.2; and whole epithelium, 16.9. The mean values for G‐6‐PDH activity were: surface layer, 10.9; granular layer, 34.4; spinous 40.6; basal 28.7; connective tissue, 11.6; and whole epithelium, 29.7. The results are compatible with the pattern of enzyme activity previously reported for primate skin (lm and Adachi, 1966) and rat palate (Gerson. 1967).