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An ADP‐Ribosylation‐Factor(ARF)‐Like Protein involved in Regulated Secretion

1997; Wiley; Volume: 246; Issue: 2 Linguagem: Inglês

10.1111/j.1432-1033.1997.00388.x

1432-1033

Christine Icard‐Liepkalns, Philippe Ravassard, Vis A. Liepkalns, Fabienne Chatail, Jacques Mallet,

Lipid Membrane Structure and Behavior

A rat ADP-ribosylation factor(ARF)-like protein named ARL184 was identified by cDNA cloning. The corresponding recombinant protein had an apparent molecular mass of 22,000. The deduced amino acid sequence had 55% identity with the human ARL1 and four functional GTP-binding sites. Immunofluorescent confocal microscopy studies showed that ARL184 was present in the cytosol as well as in the Golgi apparatus, raising the possibility that it has a role in a secretory pathway. The involvement of this ARF-like protein in secretion was confirmed by demonstrating that ARL184 potentiated acetylcholine release in stably transfected PC12 cells. Collectively these results suggest that this ARL protein is a component of a regulated secretory pathway involved in Ca2(+)-dependent release of acetylcholine.