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Determination of Phytohormones of Environmental Impact by Capillary Zone Electrophoresis

2004; American Chemical Society; Volume: 52; Issue: 6 Linguagem: Inglês

10.1021/jf0305377

1520-5118

Antonio Segura‐Carretero, Carmen Cruces‐Blanco, M. Soriano Peña, S. Cortacero Ramírez, Alberto Fernández‐Gutiérrez,

Spectroscopy and Chemometric Analyses

A test mixture of five phytohormones [naphthaleneacetic acid (NAA), naphthoxyacetic acid (NOA), indoleacetic acid (IAA), indolebutyric acid (IBA), and indolepropionic acid (IPA)] was investigated. These compounds were cleanly separated with good resolution by capillary zone electrophoresis with a UV diode array detector using 20 mM sodium phosphate buffer (pH 7.25). The lowest detection limit was obtained for IPA (0.45 mg L(-)(1) or 0.005 mg kg(-)(1)) and the highest for NAA (1.04 mg L(-)(1) or 0.014 mg kg(-)(1)). The method has been applied for tomato samples fortified with the five phytohormones using a liquid-liquid extraction procedure, obtaining recovery percentages ranging from 91 to 109.0%.