Prostaglandin synthesis by lymphoid tissue of mice experiencing a graft-versus-host reaction: Relationship to immunosuppression
1980; Elsevier BV; Volume: 50; Issue: 2 Linguagem: Inglês
10.1016/0008-8749(80)90282-8
ISSN1090-2163
AutoresWayne S. Lapp, MARIZA L. MENDES, Holger Kirchner, Diethard Gemsa,
Tópico(s)Neuropeptides and Animal Physiology
ResumoStudies were carried out on the induction of PGE synthesis during the GVH reaction and its role in GVH-induced immunosuppression. The results demonstrated that spleen, lymph node cells and, to a much lesser degree, thymus cells obtained from adult C57BL/6 × AF1 mice treated with 50–75 × 106 C57BL/6 lymphoid cells were stimulated to produce PGE during the course of the GVH reaction. The spleen and lymph node PGE production peaked at Day 9 post-GVH induction (30- and 15-fold higher than normal, respectively). Thereafter, it declined to near normal levels by Days 25–30 post-GVH induction. Passage of GVH spleen cells through a rayon column removed macrophages but not mitogen-responsive T and B cells and also removed nearly all of the PGE-producing cells, except during the later course of the GVH reaction. Removal of PGE-producing cells from GVH-immunosuppressed spleen cells significantly reconstituted the mitogen response to PHA and LPS. Treatment of mice experiencing a GVH reaction with indomethacin delayed the onset of suppression of the plaque-forming cell response to sheep erythrocytes. These results suggest that early GVH-induced immunosuppression which may represent an amplified normal regulatory mechanism is mediated by increased macrophage production of PGE which suppresses both B- and T-cell functions, whereas at later stages other immunosuppressive mechanisms become operational.
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