Placental-type alkaline phosphatase m pregnancy and malignancy plasma: specific estimation using a monoclonal antibody in a solid phase enzyme immunoassay
1983; Elsevier BV; Volume: 130; Issue: 2 Linguagem: Inglês
10.1016/0009-8981(83)90117-1
ISSN1873-3492
AutoresP. J. McLaughlin, Henry Gee, Peter Johnson,
Tópico(s)Celiac Disease Research and Management
ResumoA solid-phase enzyme immunoassay has been developed using a monoclonal antibody with specificity only for the placental-type alkaline phosphatase isoenzyme (P1-ALP). This assay is uniquely sensitive, having a lower limit of detection of 0.1 U/l (approximately 0.07 μug/1) P1-ALP. All plasma samples from healthy non-pregnant individuals had undetectable P1-ALP levels whereas, in pregnancy, a wide range (0–400 U/1) of P1-ALP levels was noted for 208 maternal plasma samples from 12 weeks gestation to term. There was no significant difference in circulating P1-ALP levels between severe pre-eclamptic and normal pregnancy. Low levels of P1-ALP (0.1–4.7 U/1) were found in 31 of 217 plasmas from human malignancies. Within these, 23 of 65 (35%) histologically-proven ovarian carcinoma patients were positive.
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