EXPRESSION IN THE METHYLOTROPHIC YEAST Pichia pastoris
1999; Elsevier BV; Linguagem: Inglês
10.1016/b978-012253840-7/50007-9
Autores Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoThe pichia pastoris expression system has gained acceptance as an important host organism for the production of foreign proteins. This chapter provides a basic understanding of the P. pastoris expression system and deals with various questions related to it, essentially describing how this system works. The chapter explains the proteins that are most likely to work well in the system, the problems to anticipate while using it, and potential solutions. The chapter discusses the success of the P. pastoris system and exclaims that opportunities still exist to increase the range of proteins that can be expressed in the system. The discussion concludes with the fact that studies are required to address problems associated with the secretion of mammalian proteins from P. pastoris . There are certain questions that need to be resolved, which require further study of this expression system. Thus, a better understanding of P. pastoris proteases and, in particular, the identification of those responsible for the degradation of some foreign proteins would aid in the development of strategies to eliminate or circumvent their effects.
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