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Retracted: Long-Term Maintenance of Undifferentiated Human Embryonic and Induced Pluripotent Stem Cells in Suspension

2011; Mary Ann Liebert, Inc.; Volume: 20; Issue: 11 Linguagem: Inglês

10.1089/scd.2010.0517

1557-8534

Mehran Rezaei Larijani, Ali Seifinejad, Behshad Pournasr, Vahid Hajihoseini, Seyedeh‐Nafiseh Hassani, Mehdi Totonchi, Maryam Yousefi, Farnaz Shamsi, Ghasem Hosseini Salekdeh, Hossein Baharvand,

3D Printing in Biomedical Research

Stem Cells and Development officially retracts the paper entitled, "Long-Term Maintenance of Undifferentiated Human Embryonic and Induced Pluripotent Stem Cells in Suspension," by Mehran Rezaei Larijani, Ali Seifinejad, Behshad Pournasr, Vahid Hajihoseini, Seydeh-Nafiseh Hassani, Mehdi Totonchi, Maryam Yousefi, Farnaz Shamsi, Ghasem Hosseini Salekdeh, and Hossein Baharvand (Stem Cells Dev 2011; 20(11):1911–1923) due to concerns regarding replicated panels within Figure 3. The Editor-in-Chief of Stem Cells and Development invited the corresponding author, Dr. Hossein Baharvand, to respond to the issues raised. Dr. Baharvand's response was as follows: "The experiment related to Figure 3A has been performed more than 10 years ago in our laboratory and the paper has been published in 2011. Unfortunately, at this point, we could not retrieve the original gel images to investigate the source of this possible error. However, we stand by our statement regarding the maintained differentiation potential of these pluripotent stem cell lines under three-dimensional culture. Pluripotency was also separately confirmed with directed differentiation and expression of the markers in Figures 3B and 3C. Furthermore, the differentiation potential of these suspension-expanded human pluripotent stem cell lines has been shown in our other studies.1–7 Importantly, our conclusions remain unchanged regarding human pluripotent stem cell differentiation potential into three-germ layer derivatives using the proposed method. Finally, the data in figure 3A has no bearing on the main conclusion of our manuscript, that is our approach maintained the capability of freeze/thawing, karyotype stability, transcriptome, and pluripotency of hESCs and hiPSCs when expanded in stirred bioreactors. The authors apologize to the community for the inconvenience this has caused and request that our article to be retracted to avoid any potential ambiguities in the future." Based on this explanation, the Editor of Stem Cells and Development agrees that the article requires retraction. The Editor and Publisher of Stem Cells and Development are committed to preserving the scientific literature and the community it serves. 1. Vosough M, Omidinia E, Kadivar M, Shokrgozar M-A, Pournasr B, Aghdami N, and Baharvand H. (2013). Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture. Stem Cells Dev 22:2693–2705. 2. Abbasalizadeh S, Larijani MR, Samadian A, and Baharvand H. (2012). Bioprocess development for mass production of sizecontrolled human pluripotent stem cell aggregates in stirred suspension bioreactor. Tissue Eng Part C Methods 18:831–851. 3. Farzaneh Z, Najarasl M, Abbasalizadeh S, Vosough M, and Baharvand H. (2017). Developing a cost-effective and scalable production of human hepatic competent endoderm from size-controlled pluripotent stem cell aggregates. Stem Cells Dev 27(4):262–274. 4. Heidariyan Z, Ghanian MH, Ashjari M, Farzaneh Z, Najarasl M, Larijani MR, Piryaei A, Vosough M, and Baharvan H. (2018). Efficient and cost-effective generation of hepatocyte-like cells through microparticle-mediated delivery of growth factors in a 3D culture of human pluripotent stem cells. Biomaterials 159:174–188. 5. Varzideh F, Pahlavan S, Ansari H, Halvaei M, Kostin S, Feiz M-S, Latifi H, Aghdami N, Braun T, Baharvand H. (2019). Human cardiomyocytes undergo enhanced maturation in embryonic stem cell-derived organoid transplants. Biomaterials 192:537–550. 6. Fonoudi H, Ansari H, Abbasalizadeh S, Larijani MR, Kiani S, Hashemizadeh S, Zarchi AS, Bosman A, Blue GM, Pahlavan S, Perry M, Orr Y, Mayorchak Y, Vandenberg J, Talkhabi M, Winlaw DS, Harvey RP, Aghdami N, and Baharvand H. (2015). A universal and robust integrated platform for the scalable production of human cardiomyocytes from pluripotent stem cells. Stem Cells Transl Med 4(12):1482–94. 7. Vahdat S, Pahlavan S, Mahmoudi E, Barekat M, Ansari H, Bakhshandeh B, Aghdami N, and Baharvand H. (2019). Expansion of human pluripotent stem cell-derived early cardiovascular progenitor cells by a cocktail of signaling factors. Sci Rep 9(1):16006.