Perinatal lethality (ple): A mutation caused by integration of a transgene into distal mouse chromosome 15
1989; Elsevier BV; Volume: 4; Issue: 4 Linguagem: Inglês
10.1016/0888-7543(89)90272-3
ISSN1089-8646
AutoresDavid R. Beier, Cynthia C. Morton, Aya Leder, Racheal Wallace, Philip Leder,
Tópico(s)Pluripotent Stem Cells Research
ResumoWe have used cytogenetic and recombinational analysis to determine the position of a transgene integrated into the mouse genome. The transgene maps to band F on the physical map of mouse chromosome 15 by in situ analysis and is tightly linked genetically to a cluster of loci that include the mutations caracul (Ca) and microcytic anemia (mk). Genetic analysis of the offspring of noninbred animals carrying the transgene and marker loci demonstrates a significant deficiency of homozygous progeny at weaning. When inbred mice heterozygous for the transgene are mated, about one-quarter of their offspring are homozygous; none of these animals survives more than 1 day after birth. It appears likely that a recessive insertional mutation has occurred as a result of transgene integration into a locus required for postnatal viability. We call this mutation transgenic perinatal lethality (Tg.ple).
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