Complete Amino‐Acid Sequence of a Non‐neurotoxic, Non‐enzymatic Phospholipase A 2 Homolog from the Venom of the Australian Tiger Snake Notechis scutatus scutatus
1980; Wiley; Volume: 111; Issue: 2 Linguagem: Inglês
10.1111/j.1432-1033.1980.tb04954.x
ISSN1432-1033
Autores Tópico(s)Biochemical and Structural Characterization
ResumoThe complete amino acid sequence of Notechis II-1, a non-neurotoxic, non-enzymatic phospholipase A2 homolog from the venom of the Australian tiger snake Notechis s. scutatus has been determined. The protein consists of a single chain of 119 amino acids. The main fragmentation of the reduced and S-carboxymethylated derivative was accomplished by cleavage with Staphylococcus aureus V8 protease. Tryptic peptides were used to align and complete the sequence, which was determined mainly by automated Edman degradation. Notechis II-1 contains all of the residues that appear to be invariant in elapid and pancreatic phospholipases A2 except at position 30 in the sesquence, where an otherwise invariant glycine is replaced by serine.
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