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BIBTEX RIS

Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses

2010; Springer Science+Business Media; Volume: 156; Issue: 2 Linguagem: Inglês

10.1007/s00705-010-0857-z

1432-8798

Rafael K. Campos, Mário Celso Sperotto Brum, Carlos Eduardo Wayne Nogueira, Betânia Paiva Drumond, Pedro Augusto Alves, Larissa Siqueira-Lima, Felipe Lopes de Assis, Giliane S. Trindade, Cláudio Antônio Bonjardim, P. C. P. Ferreira, Rudi Weiblen, Eduardo Furtado Flores, Erna Geessien Kroon, Jônatas Santos Abrahão,

Virus-based gene therapy research

During the last bovine vaccinia (BV) outbreaks, several Vaccinia virus (VACV) strains were isolated and characterised, revealing significant polymorphisms between strains, even within conserved genes. Although the epidemiology of VACV has been studied in BV outbreaks, there is little data about the circulation of the Brazilian VACV isolates. This study describes the genetic and biological characterisation of two VACV isolates, Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), which were obtained concomitantly from a horse affected by severe cutaneous disease. Despite being isolated from the same exanthematic clinical sample, P1V and P2V showed differences in their plaque phenotype and in one-step growth curves. Moreover, P1V and P2V presented distinct virulence profiles in a BALB/c mouse model, as observed with other Brazilian VACV isolates. Sequencing and phylogenetic analysis of four different genes demonstrated that the isolates are segregated in different VACV clusters. Our results raise interesting questions about the diversity of VACV isolates in Brazil.