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Demonstration of identical expanded clones within both CD8+ CD28+ and CD8+ CD28− T cell subsets in HIV type 1-infected individuals

1998; Wiley; Volume: 28; Issue: 5 Linguagem: Inglês

10.1002/(sici)1521-4141(199805)28

1521-4141

Emiliano N. Mugnaini, Anne Spurkland, Thore Egeland, Mette Sannes, Jan E. Brinchmann,

Cytomegalovirus and herpesvirus research

In HIV-1-infected individuals, the CD8+ CD28− T cell subset is considerably expanded and is frequently the largest subset of T cells found in peripheral blood. It has been assumed, but not proven, that CD8+ CD28− T cells derive from CD8+ CD28+ T cells in vivo. To further study the ontogeny of CD8+ CD28− T cells, we have performed analyses of the complementarity determining region 3 (CDR3) of the TCRB of CD8+ CD28+ and CD8+ CD28− T cells from the peripheral blood of HIV-1-infected individuals. When cells from the same individual were compared, expanded peaks in CDR3 length analysis within a given BV family were frequently observed at the same location in both CD8+ subsets ( p < 0.001). Sequencing of cDNA corresponding to dominant peaks revealed the presence of identical expanded CD8+ T cell clones within both the CD28+ and CD28− subsets on eight of nine attempts. Our results show that CD8+ CD28+ and CD8+ CD28− T cells are phenotypic variants of the same lineage, most likely evolving from CD8+ CD28+ to end-stage CD8+ CD28− T cells.