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Liquid chromatographic and mass spectrometric analysis of human serum acid alpha‐1‐glycoprotein

2004; Wiley; Volume: 18; Issue: 5 Linguagem: Inglês

10.1002/bmc.324

1099-0801

T. Kremmer, Eva Szöllösi, M. Boldizsár, B. Vincze, Krisztina Ludányi, Tı́mea Imre, Gitta Schlosser, Károly Vékey,

Advanced Proteomics Techniques and Applications

Human serum acid alpha-1-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls.