Portal de Periódicos da CAPES

Unexpected Inhibition of Peptidoglycan LD-Transpeptidase from Enterococcus faecium by the β-Lactam Imipenem

2007; Elsevier BV; Volume: 282; Issue: 42 Linguagem: Inglês

10.1074/jbc.m704286200

1083-351X

Jean‐Luc Mainardi, Jean‐Emmanuel Hugonnet, Filippo Rusconi, Martine Fourgeaud, Lionel Dubost, Angèle Nguekam Moumi, V. Delfosse, Claudine Mayer, Laurent Gutmann, Louis B. Rice, Michel Arthur,

Pneumonia and Respiratory Infections

The β-lactam antibiotics mimic the d-alanyl 4 -d-alanine 5 extremity of peptidoglycan precursors and act as "suicide" substrates of the dd-transpeptidases that catalyze the last cross-linking step of peptidoglycan synthesis. We have previously shown that bypass of the dd-transpeptidases by the ld-transpeptidase of Enterococcus faecium (Ldt fm ) leads to high level resistance to ampicillin. Ldt fm is specific for the l-lysyl 3 -d-alanine 4 bond of peptidoglycan precursors containing a tetrapeptide stem lacking d-alanine 5 . This specificity was proposed to account for resistance, because the substrate of Ldt fm does not mimic β-lactams in contrast to the d-alanyl 4 -d-alanine 5 extremity of pentapeptide stems used by the dd-transpeptidases. Here, we unexpectedly show that imipenem, a β-lactam of the carbapenem class, totally inhibited Ldt fm at a low drug concentration that was sufficient to inhibit growth of the bacteria. Peptidoglycan cross-linking was also inhibited, indicating that Ldt fm is the in vivo target of imipenem. Stoichiometric and covalent modification of Ldt fm by imipenem was detected by mass spectrometry. The modification was mapped into the trypsin fragment of Ldt fm containing the catalytic Cys residue, and the Cys to Ala substitution prevented imipenem binding. The mass increment matched the mass of imipenem, indicating that inactivation of Ldt fm is likely to involve rupture of the β-lactam ring and acylation of the catalytic Cys residue. Thus, the spectrum of activity of β-lactams is not restricted to transpeptidases of the dd-specificity, as previously thought. Combination therapy with imipenem and ampicillin could therefore be active against E. faecium strains having the dual capacity to manufacture peptidoglycan with transpeptidases of the ld- and dd-specificities.