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Simultaneous determination of benzodiazepines in whole blood or serum by HPLC/DAD with a semi-micro column

2000; Elsevier BV; Volume: 23; Issue: 2-3 Linguagem: Inglês

10.1016/s0731-7085(00)00325-3

1873-264X

Anissa El Mahjoub, Christian Staub,

Neuroscience and Neuropharmacology Research

A simple and sensitive high performance liquid chromatographic (HPLC) method using a semi-micro column, C8 reversed-phase column (3 mm i.d.) and a low flow rate 0.3 ml/min was developed and validated for the determination of five frequently prescribed benzodiazepines: clonazepam, diazepam, flunitrazepam, midazolam and oxazepam. Quantification was performed at 220 nm with methylclonazepam as internal standard. The method involved a simple extraction from alkalinized blood (1 ml) into 1-chlorobutane and provided excellent sensitivity, recovery, accuracy and reproducibility for benzodiazepines in therapeutic or toxic concentrations.