Crippling of CD3-ζ ITAMs Does Not Impair T Cell Receptor Signaling
1999; Cell Press; Volume: 10; Issue: 4 Linguagem: Inglês
10.1016/s1074-7613(00)80041-2
ISSN1097-4180
AutoresLaurence Ardouin, Claude Boyer, A. Gillet, Jeannine Trucy, Anne‐Marie Bernard, Jacques A. Nunès, Jérôme Delon, Alain Trautmann, Hai‐Tao He, Bernard Malissen, Marie Malissen,
Tópico(s)Synthesis and Biological Evaluation
ResumoWe evaluated the importance of CD3-ζ ITAMs in T cell responses by breeding the P14 transgenic TCR into mice in which CD3-ζ chains lacking all or part of their ITAMs were genetically substituted for wild-type CD3-ζ chains. In contrast to the H-Y TCR, the P14 TCR permitted the development of peripheral CD8+ T cells harboring signaling-defective CD3-ζ subunits. The absence of functional CD3-ζ ITAMs did not reduce the spectrum of activation events and effector functions that constitute the normal attributes of mature CD8+ T cells. The only detectable differences were quantitative and noted only when T cells were challenged with suboptimal peptide concentrations. Therefore, the ITAMs present in the CD3-γδε module are sufficient for qualitatively normal TCR signaling and those present in CD3-ζ have no exclusive role during T cell activation.
Referência(s)