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Cellular organization and appearance of differentiated structures in developing stages of the parasitic platyhelminth Echinococcus granulosus

2004; Wiley; Volume: 94; Issue: 2 Linguagem: Inglês

10.1002/jcb.20294

1097-4644

Claudio Martı́nez, Rodolfo Paredes, Roberto P. Stock, Andrés Saralegui, Manuel J. Andreu, Carolina Cabezón, Ricardo Ehrlich, Norbel Galanti,

Parasitic Infections and Diagnostics

Journal of Cellular BiochemistryVolume 94, Issue 2 p. 327-335 Article Cellular organization and appearance of differentiated structures in developing stages of the parasitic platyhelminth Echinococcus granulosus Claudio Martínez, Corresponding Author Claudio Martínez clau@fcien.edu.uy Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, UruguaySección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, Uruguay.Search for more papers by this authorR. Paredes, R. Paredes Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorR.P. Stock, R.P. Stock Instituto de Biotecnología, U.N.A.M., Av. Universidad 2001, Cuernavaca, Morelos, México, C.P. 62170Search for more papers by this authorA. Saralegui, A. Saralegui Instituto de Biotecnología, U.N.A.M., Av. Universidad 2001, Cuernavaca, Morelos, México, C.P. 62170Search for more papers by this authorM. Andreu, M. Andreu Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorC. Cabezón, C. Cabezón Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorR. Ehrlich, R. Ehrlich Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, UruguaySearch for more papers by this authorN. Galanti, N. Galanti Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this author Claudio Martínez, Corresponding Author Claudio Martínez clau@fcien.edu.uy Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, UruguaySección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, Uruguay.Search for more papers by this authorR. Paredes, R. Paredes Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorR.P. Stock, R.P. Stock Instituto de Biotecnología, U.N.A.M., Av. Universidad 2001, Cuernavaca, Morelos, México, C.P. 62170Search for more papers by this authorA. Saralegui, A. Saralegui Instituto de Biotecnología, U.N.A.M., Av. Universidad 2001, Cuernavaca, Morelos, México, C.P. 62170Search for more papers by this authorM. Andreu, M. Andreu Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorC. Cabezón, C. Cabezón Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this authorR. Ehrlich, R. Ehrlich Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP1400, Montevideo, UruguaySearch for more papers by this authorN. Galanti, N. Galanti Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, ChileSearch for more papers by this author First published: 03 November 2004 https://doi.org/10.1002/jcb.20294Citations: 20Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Echinococcus granulosus is the causative agent of hydatidosis, a major zoonoses that affects humans and herbivorous domestic animals. The disease is caused by the pressure exerted on viscera by hydatid cysts that are formed upon ingestion of E. granulosus eggs excreted by canine. Protoscoleces, larval forms infective to canine, develop asynchronously and clonally from the germinal layer (GL) of hydatid cysts. In this report, we describe the cellular organization and the appearance of differentiated structures both in nascent buds and developed protoscoleces attached to the GL. Early protoscolex morphogenesis is a highly complex and dynamic process starting from the constitution of a foramen in the early bud, around which nuclei are distributed mainly at the lateral and apical regions. Similarly, distribution of nuclei in mature protoscoleces is not homogenous but underlies three cellular territories: the suckers, the rostellar pad, and the body, that surrounds the foramen. Several nuclei are associated to calcareous corpuscles (Cc), differentiated structures that are absent in the earlier bud stages. The number of nuclei is similar from the grown, elongated bud stage to the mature protoscolex attached to the GL, strongly suggesting that there is no significant cellular proliferation during final protoscolex development. The amount of DNA per nucleus is in the same range to the one described for most other platyhelminthes. Our results point to a sequential series of events involving cell proliferation, spatial cell organization, and differentiation, starting in early buds at the GL of fertile hydatid cysts leading to mature protoscoleces infective to canine. © 2004 Wiley-Liss, Inc. Citing Literature Volume94, Issue21 February 2005Pages 327-335 RelatedInformation