Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy
2000; Optica Publishing Group; Volume: 25; Issue: 1 Linguagem: Inglês
10.1364/ol.25.000052
ISSN1539-4794
AutoresO. Albert, L. Sherman, G. Mourou, Theodore B. Norris, Gleb Vdovin,
Tópico(s)Optical Coherence Tomography Applications
ResumoOff-axis aberrations in a beam-scanning multiphoton confocal microscope are corrected with a deformable mirror. The optimal mirror shape for each pixel is determined by a genetic learning algorithm, in which the second-harmonic or two-photon fluorescence signal from a reference sample is maximized. The speed of the convergence is improved by use of a Zernike polynomial basis for the deformable mirror shape. This adaptive optical correction scheme is implemented in an all-reflective system by use of extremely short (10-fs) optical pulses, and it is shown that the scanning area of an f:1 off-axis parabola can be increased by nine times with this technique.
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