Determination of tadalafil and N -desmethylsibutramine in health and dietary supplements using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS)
2011; Taylor & Francis; Volume: 28; Issue: 11 Linguagem: Inglês
10.1080/19440049.2011.601280
ISSN1944-0049
AutoresSoung‐Hun Roh, Yun Pyo Kang, Susan Park, Youngsook Huh, Jeongmi Lee, J.H. Park, D. Kim, Sung Won Kwon,
Tópico(s)Hormonal and reproductive studies
ResumoThe adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 µg kg−1 and from 1.3 to 6.0 µg kg−1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.
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