Rapid preparative separation of natural products by centrifugal thin-layer chromatography
1980; Elsevier BV; Volume: 202; Issue: 1 Linguagem: Inglês
10.1016/s0021-9673(00)80093-x
ISSN1873-3778
AutoresKurt Hostettmann, Maryse Hostettmann‐Kaldas, Otto Sticher,
Tópico(s)Mycotoxins in Agriculture and Food
ResumoConventional layer liquid chromatographic techniques—practically, from paper chromatography through thin-layer chromatography (TLC) to high-performance thin-layer chromatography (HPTLC)—illustrated the unique potentialities of layer liquid systems. Formerly, these technical solutions based further advanced directions on layer liquid chromatography (LLC). However, the development of special adsorbent layers and eluent systems as well as separation chambers for TLC/HPTLC developments demanded innovative steps in chemical-instrumental technologies. Partial renewal of TLC (development of HPTLC) decreased the dimensions of the basic TLC and represents a certain progress in LLC. The automation and hyphenation, e.g., with modern spectroscopic techniques [such as TLC/HPTLC-MS (mass spectroscopy) or TLC/HPTLC-FTIR (Fourier transform infrared)] as well as multidimensional separations, illustrate the future horizons and further potential in LLC. The drawbacks of HPTLC (e.g., limited preparative applicability, limited spot capacity possibilities), as the most efficient technique among the conventional layer liquid chromatographic techniques, have resulted in claims for optimum eluent front velocity, which can be generated by using forced flow of the eluent.
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