Enzyme-mediated initiation of acrylamide polymerization: reaction mechanism
2000; Elsevier BV; Volume: 41; Issue: 23 Linguagem: Inglês
10.1016/s0032-3861(00)00204-4
ISSN1873-2291
Autores Tópico(s)Lanthanide and Transition Metal Complexes
ResumoThe polymerization of acrylamide in water initiated by a horseradish peroxidase-catalyzed redox system is studied. It combines hydrogen peroxide (as the oxidant) and 2,4-pentanedione (as the reductant). Several side reactions leading to the degradation of the enzyme through the formation of compound III are evidenced by polymerization experiments and UV spectroscopy; they can be avoided by adjusting the reactant concentrations. The oxidation of 2,4-pentanedione by a non-enzymic pathway is also detected by 1H NMR. Its main effect is to reduce the concentration of the enol form of 2,4-pentanedione, and hence to modify the initiation rate. From this information, a schematic picture of the reactions involved in the enzyme-catalyzed redox system is drawn, so as to optimize the polymerization conditions.
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