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NHE-1 and <meta charset="utf-8" /> β1 integrin dependent monocyte adhesion and migration after glucose, insulin or PPARγ stimulation

2011; Landes Bioscience; Volume: 5; Issue: 3 Linguagem: Inglês

10.4161/cam.5.3.14534

1933-6926

Zacharoula Zolota, George Koliakos, Konstantinos Paletas, Martha Kaloyianni,

Atherosclerosis and Cardiovascular Diseases

In the present study the effect of high glucose concentrations, insulin, PPARγ activators (rosiglitazone) and NHE-1 inhibitors (cariporide) in atherosclerosis-related functions of human monocytes was investigated. Monocyte adhesion to laminin-1, collagen type IV and endothelial cells, as well as monocyte migration through the same substrates were studied. Incubation of the monocyte suspension with high glucose concentrations, insulin and rosiglitazone induced all the studied atherosclerosis-related functions of the monocytes. In all these functions the addition of cariporide counteracted the activity of glucose, insulin and rosiglitazone. The use of antigen for β1 integrin also counteracted the activity of the above in monocyte adhesion in all three substrates. The data of the present study suggests that PPARγ activation in monocytes induces atherosclerosis, and that NHE-1 and β1 integrin play an important role in the beginning of atherosclerosis. p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px 'Lucida Grande'}