The schiff base between pyridoxal‐5′‐phosphate (PLP) and hexylamine. Formation of the unprotonated form of the imine by reaction of the unprotonated PLP and free amine
1991; Wiley; Volume: 4; Issue: 6 Linguagem: Inglês
10.1002/poc.610040609
ISSN1099-1395
AutoresManuel Blázquez, Teresa Pineda, J.M. Sevilla, M. Domı́nguez, F. García-Blanco,
Tópico(s)Analytical Chemistry and Sensors
ResumoAbstract Electrochemical and spectrophotometric studies of the pyridoxal‐5′ ‐phosphate hexylamine Schiff base (PHSB) in strongly basic media were carried out. The equilibrium constant and rate constants of the formation and hydrolysis of the unprotonated imine from the unprotonated pyridoxal‐5′ ‐phosphate and free amine were determined by linearscan cyclic voltammetry. The acid‐base dissociation constant of the imine group was obtained by spectrophotometric measurements. Absorption bands for the free imine and the conjugate acid are characterized by a log‐normal distribution. These studies are for general application to the quantitative characterization of non‐enzymatic model compounds.
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