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Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation

2012; Springer Nature; Volume: 19; Issue: 7 Linguagem: Inglês

10.1038/cdd.2011.205

1476-5403

Roberto Rizzi, Elisa Di Pasquale, Paola Portararo, Roberto Papait, Paola Cattaneo, Michael V.G. Latronico, Claudia Altomare, Luca Sala, Antonio Zaza, Emilio Hirsch, Luigi Naldini, Gianluigi Condorelli, Claudia Bearzi,

CRISPR and Genetic Engineering

Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation.