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Activation by “Feedback Regulator” and some properties of guanylate cyclase of plasma membrane of rat epididymal fat cells

1976; Elsevier BV; Volume: 72; Issue: 4 Linguagem: Inglês

10.1016/s0006-291x(76)80161-1

1090-2104

Takeo Asakawa, Constance Johnson, Juan Ruiz, Ilene Scheinbaum, Thomas Russell, Ren-jye Ho,

Receptor Mechanisms and Signaling

Low levels of FR (feedback regulator), an inhibitor of adenylate cyclase, stimulates guanylate cyclase about 10-fold. The extent of stimulation depends on FR concentration, but is reversed at higher FR levels. At lower enzyme concentrations less FR is required for maximal activation. ER-stimulated activity is different from basal activity as it is more pH and [Mn2+] dependant. Basal activity has a broad optimum at higher pH. Both basal and stimulated activities show a sigmoidal dependance on [GTP].