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EGFR gene amplification ‐ rearrangement in human glioblastomas

1995; Wiley; Volume: 62; Issue: 2 Linguagem: Inglês

10.1002/ijc.2910620206

1097-0215

K. Schwechheimer, Su Huang, Webster K. Cavenee,

RNA Research and Splicing

Abstract Immunostaining using an affinity‐purified rabbit polyclonal antibody against the extracellular domain of the epidermal‐growth‐factor receptor (EGFR) showed over‐expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc.