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Amino acid racemization in amber-entombed insects: Implications for DNA preservation

1994; Elsevier BV; Volume: 58; Issue: 14 Linguagem: Inglês

10.1016/0016-7037(94)90185-6

1872-9533

Jeffrey L. Bada, Xueyun S. Wang, H. N. Poinar, Svante Pääbo, George O. Poinar,

Insect Resistance and Genetics

DNA depurination and amino acid racemization take place at similar rates in aqueous solution at neutral pH. This relationship suggests that amino acid racemization may be useful in accessing the extent of DNA chain breakage in ancient biological remains. To test this suggestion, we have investigated the amino acids in insects entombed in fossilized tree resins ranging in age from 10(4). These results suggest that in amber insect inclusions DNA depurination rates would also likely be retarded in comparison to aqueous solution measurements, and thus DNA fragments containing many hundreds of base pairs should be preserved. This conclusion is consistent with the reported successful retrieval of DNA sequences from amber-entombed organisms.