Partial sequence analysis of Xenopus α- and β-globin mRNA as determined from recombinant DNA plasmids
1980; Elsevier BV; Volume: 78; Issue: 1 Linguagem: Inglês
10.1016/0012-1606(80)90326-7
ISSN1095-564X
AutoresCharles C. Richardson, Joe Cappello, Mark D. Cochran, Richard W. Armentrout, Ronald D. Brown,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoRecombinant plasmids containing Xenopus globin mRNA sequences have been constructed using the mRNA:cDNA hybrid conditions of Zain et al. (1979, Cell 16, 851–861). The partial nucleotide sequence of two of these recombinants has been determined. They have been identified as containing α- and β-globin-like sequences by homology to other amphibian globin proteins. The nucleotide sequence of these recombinants permits the comparison of conserved regions in both the coding and 3′ nontranslated regions of Xenopus globin mRNAs with the known sequences of other eukaryotic globin proteins and mRNAs. Among the features which have been conserved though evolution is the sequence AAUAAA close to the 3′ terminus of the nontranslated region. Extensive regions of homology occur between the 3′ nontranslated regions of Xenopus α- and β-globin mRNA.
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