Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates
2007; Elsevier BV; Volume: 97; Issue: 2 Linguagem: Inglês
10.1016/j.jip.2007.07.003
ISSN1096-0805
AutoresPhilippe Blanchard, Violaine Olivier, Anne-Laure Iscache, Olivier Celle, Frank M. Schurr, Perrine Lallemand, Magali Ribière,
Tópico(s)Plant Virus Research Studies
ResumoA new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.
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