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Novel reactions of xylose isomerase from Streptomyces rubiginosus

1999; Elsevier BV; Volume: 25; Issue: 8-9 Linguagem: Inglês

10.1016/s0141-0229(99)00100-3

1879-0909

Ossi Pastinen, Kalevi Visuri, Hans E. Schoemaker, Matti Leisola,

Pancreatic function and diabetes

Three novel substrates—d-arabinose, l-ribose, and d-lyxose—are described for the industrially important xylose isomerase (glucose isomerase). Furthermore, it was shown that contrary to previous studies, two different reaction products are formed when pentose sugars are used as substrates. In extended reactions an apparent equilibrium is formed between both d- and l-forms of arabinose, ribulose, and ribose as well as d-xylose, d-lyxose, and d-xylulose. The new reactions are discussed in the light of previously described reaction mechanisms for this enzyme.