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The effects on cell adhesion of fibronectin and gelatin in a serum-free, bovine serum albumin medium.

1982; Japan Society of Cell Biology; Volume: 7; Issue: 3 Linguagem: Inglês

10.1247/csf.7.245

1347-3700

Mikio Kan, Yoshiki Minamoto, Sachiko Sunami, Isao Yamam, Makoto Umeda,

Skin and Cellular Biology Research

Macromolecules which eliminate the effects of bovine serum albumin (BSA) to inhibit cell adhesion, were investigated using serum-free medium supplemented with 5 g/1 of BSA. Fibronectin (FN) produces adhesion in various types of cells-BHK-21, TE-1, FL, HeLa-S3 and human diploid fibroblasts, while asdoes gelatin in fibroblasts and TE-1 cells. These two types which adhered to gelatin-coated dishes secreted sufficient amounts of cellular FN into the cultured medium to allow such adhesion. The dual effects of both FN and gelatin were exhibited only when added prior to BSA. This indi-cates that further adsorption of either FN or gelatin is inhibited when BSA has already been adsorbed on the substratum. FN may also act as an adhesive bridge between cells and gelatin-coated ornon-treated dishes.