Portal de Periódicos da CAPES

Regulating genes with electromagnetic response elements

2001; Wiley; Volume: 81; Issue: 1 Linguagem: Inglês

10.1002/1097-4644(20010401)81

1097-4644

Hana Lin, Martin Blank, K Rossol-Haseroth, Reba Goodman,

Magnetic and Electromagnetic Effects

Journal of Cellular BiochemistryVolume 81, Issue 1 p. 143-148 Articles Regulating genes with electromagnetic response elements Hana Lin, Hana Lin Department of Pathology, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorMartin Blank, Martin Blank Department of Physiology, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorKarin Rossol-Haseroth, Karin Rossol-Haseroth Department of Medicine, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorReba Goodman, Corresponding Author Reba Goodman [email protected] Department of Pathology, Columbia University Health Sciences, New York, NY 10032Columbia University Health Sciences, Department of Pathology, 630 W. 168th Street, New York, NY 10032.Search for more papers by this author Hana Lin, Hana Lin Department of Pathology, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorMartin Blank, Martin Blank Department of Physiology, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorKarin Rossol-Haseroth, Karin Rossol-Haseroth Department of Medicine, Columbia University Health Sciences, New York, NY 10032Search for more papers by this authorReba Goodman, Corresponding Author Reba Goodman [email protected] Department of Pathology, Columbia University Health Sciences, New York, NY 10032Columbia University Health Sciences, Department of Pathology, 630 W. 168th Street, New York, NY 10032.Search for more papers by this author First published: 09 February 2001 https://doi.org/10.1002/1097-4644(20010401)81:1 3.0.CO;2-4Citations: 73Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract A 900 base pair segment of the c-myc promoter, containing eight nCTCTn sequences, is required for the induction of c-myc expression by electromagnetic (EM) fields. Similarly, a 70 bp region of the HSP70 promoter, containing three nCTCTn sequences, is required for the induction of HSP70 expression by EM fields. Removal of the 900 base pair segment of the c-myc promoter eliminates the ability of EM fields to induce c-myc expression. Similarly, removal of the 70 bp region of the HSP70 promoter, with its three nCTCTn sequences, eliminates the response to EM fields. The nCTCTn sequences apparently act as electromagnetic field response elements (EMRE). To test if introducing EMREs imparts the ability to respond to applied EM fields, the 900 bp segment of the c-myc promoter (containing eight EMREs) was placed upstream of CAT or luciferase reporter constructs that were otherwise unresponsive to EM fields. EMREs-reporter constructs were transfected into HeLa cells and exposed to 8 μT 60 Hz fields. Protein extracts from EM field-exposed transfectants had significant increases in activity of both CAT and luciferase, compared with identical transfectants that were sham-exposed. Transfectants with CAT or luciferase constructs lacking EMREs remained unresponsive to EM fields, i.e., there was no increase in either CAT or luciferase activity. These data support the idea that EMREs can be used as switches to regulate exogenously introduced genes in gene therapy. J. Cell. Biochem. 81:143–148, 2001. © 2001 Wiley-Liss, Inc. REFERENCES Blank M, Goodman R. 1999. Electromagnetic fields may act directly on DNA. J Cell Biochem 75: 369– 374. Goodman R, Blank M. 1998. Magnetic field stress induces expression of hsp70. Cell Stress Chaperones 3: 79– 88. Han L, Lin H, Head M, Jin M, Blank M, Goodman R. 1998. Application of magnetic field-induced hsp70 for pre-surgical cytoprotection. J Cell Biochem 71: 577– 583. Jim M, Lin H, Han L, Opler M, Maurer S, Blank M, Goodman R. 1997. Biological and technical variables in myc expression in HL60 cells exposed to 60 Hz electromagnetic fields. Bioelectrochem Bioenerg 44: 210– 217. Lin H, Goodman R, Henderson AS. 1994. Specific region of the c-myc promoter is responsive to electric and magnetic fields. J Cell Biochem 54: 281– 288. Lin H, Opler M, Head M, Blank M, Goodman R. 1997. Electromagnetic field exposure induces rapid, transitory heat shock factor activation in human cells. J Cell Biochem 66: 482– 488. Lin H, Head M, Blank M, Han L, Jin M, Goodman R. 1998a. Myc-mediated transactivation of HSP70 expression following exposure to magnetic fields. J Cell Biochem 69: 181– 188. Lin H, Head M, Blank M, Han L, Goodman R. 1998b. Magnetic field activation of protein-DNA binding. J Cell Biochem 70: 297– 303. Lin H, Blank M, Goodman R. 1999. Magnetic field-responsive domain in the human HSP70 promoter. J Cell Biochem 75: 170– 176. Meggers E, Michel-Beryerle ME, Giese B. 1998. Sequence dependent long range hole transport in DNA. J Am Chem Soc 120: 12950– 12955. Olivares-Banuelos T, Verdugo-Diaz L, Navarro L, Merez MAQ, Drucker-Colin R. 2000. Changes in gene expression induced by elf mf in differentiated chromaffin cells. Bioelectrochem Bioenerg (in press). Porath D, Berzyadin A, de Vries S, Dekker C. 2000. Direct measurement of electrical transport through DNA molecules. Nature 403: 635– 638. Verdugo-Diaz L, Feria-Velasco A, Orozco-Suarez S, Drucker-Colin R. 2000. Low frequency magnetic field stimulation in nigro-striatal lesioned rats with chromaffin cell transplants induces changes in the subventricular zone. Proc Natl Acad Sci USA (in press). Wan C, Fiebig T, Kelley SO, Treadway CR, Barton JK. 1999. Femtosecond dynamics of DNA-mediated electron transfer. Proc Nat Acad Sci USA 96: 6014– 6019. Citing Literature Volume81, Issue11 April 2001Pages 143-148 ReferencesRelatedInformation