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Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection

2002; Wiley; Volume: 16; Issue: 3 Linguagem: Inglês

10.1002/bmc.125

1099-0801

Steffen Bauer, Elke Störmer, R. Kaiser, Pierre‐Benoit Tremblay, Jürgen Brockmöller, Ivar Roots,

Anesthesia and Pain Management

A rapid and sensitive HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5-HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liquid-liquid extraction of human plasma with dichloromethane coupled with reversed-phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra- and inter-assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters.