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Establishment and characterization of a primitive neuroectodermal tumor of bone continuous cell line (LAP‐35)

1990; Wiley; Volume: 8; Issue: 6 Linguagem: Inglês

10.1002/stem.5530080644

0737-1454

Gian Paolo Bagnara, Marco Giovannini, Massimo Serra, Piero Picci, Mario Campanacci, Manuela Badiali, Donatella Granchi, Paolo Paolucci, Paola Rocchi, Andrea Pession, G Paolucci, Mario Stella, A. Montaldi, Lino Tessarollo, Andrea Cavazzana,

Neurofibromatosis and Schwannoma Cases

A continuous tumor cell line (LAP-35) was established from a primitive neuroectodermal tumor of bone from the right tibia of a 12-year-old female. The neural character of the cell line was documented by the spontaneous growth of neurites and by the presence of several neural markers, including neuron-specific enolase (NSE), S-100 protein, neurofilaments, chromogranin A, synaptophysin and positivity to monoclonal antibodies UJ127.11, UJ13A, UJ181.4. Cell-sorter analysis showed a high expression of nerve growth factor receptor (NGFr) and major histocompatibility complex class I-related molecules. A unique cytogenetic profile was observed, including a reciprocal chromosomal translocation (rct) 11:22 (q24;q12), typically associated with Ewing's sarcoma and neuroepithelioma, and deletion of the short arm of chromosome 1 (lp-), otherwise a feature of neuroblastoma. N-myc proto-oncogene was neither amplified nor expressed, whereas the expression of c-myc was documented by northern blot analysis. These features distinguish this new cell line from previously reported neuroectodermal cell lines, identifying LAP-35 as a unique model of a group of neural bone tumors that share characteristics of neuroblastoma as well as neuroepithelioma.