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Artigo Revisado por pares
BIBTEX RIS

Crystallization and preliminary crystallographic analysis of the importin-β–SREBP-2 complex

2003; Wiley; Volume: 59; Issue: 10 Linguagem: Inglês

10.1107/s0907444903018328

ISSN

1399-0047

Autores

Soo Jae Lee, Toshihiro Sekimoto, Eiki Yamashita, Emi Nagoshi, Atsushi Nakagawa, Hideaki Tanaka, Yoshihiro Yoneda, Tomitake Tsukihara,

Tópico(s)

Enzyme Structure and Function

Resumo

The nuclear-transport protein importin-beta mediates the nuclear import of the transcription factor SREBP-2 without requiring adaptor proteins such as importin-alpha. An importin-beta-SREBP-2 HLHZ domain complex was purified and crystallized. The crystals belong to space group P2(1)2(1)2(1) and show diffraction to at least 3.0 A resolution. The unit-cell parameters are a = 101.0, b = 113.2, c = 240.0 A. Structure determination using the MAD or SAD method is under way.

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