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ELISA inhibition method in detection of mite and chironomid antigens in environmental samples of dust, soil and air

1990; Wiley; Volume: 45; Issue: 3 Linguagem: Inglês

10.1111/j.1398-9995.1990.tb00479.x

1398-9995

Jiro Kimura, Hiroyuki Matsuoka, Akira Ishii,

Study of Mite Species

To determine the amount of chironomids and mite antigens in the indoor and outdoor environment, we investigated samples from air, soil and house dust in the area around Lake Kojima, located in the western part of Japan. Three species of chironomid ( Tokunagayusurika akamusi, Chironomus yoshimatsui and Chironomus plumosus ) and a species of mite, Dermatophagoides farinae antigens were studied. The antigens were detected and quantified by inhibition ELISA. In the outdoor environment, C. yoshimatsui and T. akamusi antigens were more abundant than mite antigen, and seasonal fluctuations were observed. In the indoor environment, mite was the predominant antigen. D. farinae antigen was detected in almost all dust samples, with a slight reduction in winter compared to summer.