Dependency plots suggest the kinetic structure of ion channels
1992; Royal Society; Volume: 249; Issue: 1325 Linguagem: Inglês
10.1098/rspb.1992.0095
ISSN1471-2954
Autores Tópico(s)Mass Spectrometry Techniques and Applications
ResumoRestricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Magleby K. L. and Song L. 1992Dependency plots suggest the kinetic structure of ion channelsProc. R. Soc. Lond. B.249133–142http://doi.org/10.1098/rspb.1992.0095SectionRestricted accessArticleDependency plots suggest the kinetic structure of ion channels K. L. Magleby Google Scholar Find this author on PubMed Search for more papers by this author and L. Song Google Scholar Find this author on PubMed Search for more papers by this author K. L. Magleby Google Scholar Find this author on PubMed and L. Song Google Scholar Find this author on PubMed Published:22 August 1992https://doi.org/10.1098/rspb.1992.0095AbstractIon channels are integral membrane proteins that regulate ionic flux through cell membranes by opening and closing (gating) their pores. The gating can be monitored by observing step changes in the current flowing through single channels, and analysis of the observed open and closed interval durations has provided a window to develop kinetic models for the gating process. One difficulty in developing such models has been to determine the connections (transition pathways) among the various kinetic states involved in the gating. To help overcome this difficulty we present a transform (dependency plot) of the single-channel data that can give immediate insight into the connections. A dependency plot is derived by calculating a contingency table from a two-dimensional (joint density) dwell-time distribution of adjacent open and closed intervals by assuming that the two classified criteria are the open and closed durations of each pair of adjacent intervals. A three-dimensional surface plot of the fractional difference between the numbers of observed interval pairs and the numbers expected if the durations of adjacent intervals are independent then gives the dependency plot. An excess of interval pairs in the dependency plot suggests that the open and closed states (or compound states) that give rise to the interval pairs in excess are directly connected. A deficit of interval pairs suggests that the open and closed states (or compound states) that give rise to the interval pairs in deficit are either not directly connected or that there are additional open–closed transition pathways arising from the directly connected states.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. 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