Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of human salivary proteins including pink‐violet components: Range, distribution and response to 2‐mercaptoethanol
1987; Wiley; Volume: 8; Issue: 12 Linguagem: Inglês
10.1002/elps.1150081208
ISSN1522-2683
AutoresT. C. Marshall, Katherine M. Williams,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoAbstract Mid‐morning specimens of unstimulated whole saliva were collected from 160 apparently healthy individuals and analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis following sample preparation with or without 2‐mercaptoethanol. Coomassie Brilliant Blue R‐250 staining revealed blue and pink‐violet salivary protein components. The latter, detected in approximately 60 % of the individuals, formed a limited range of patterns combining 2–8 of 11 diffuse zones of M r 10 000–100 000. The pink‐violet components were unaffected by 2‐mercaptoethanol, urea or prior centrifugation of the sample and were not invariably present or absent in repeat samples from the same individual. The patterns of the blue staining components were more reproducible for the purpose of human individualisation but were affected by 2‐mercaptoethanol or prior centrifugation of the sample. The patterns of both the blue and pink‐violet components were not affected by sample storage at −70 °C. Only 5 μL of saliva were required for analysis giving excellent resolution over the M r range 10 000–500 000.
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