Isolation of Melanosomes from Keratinous Material – A New Method
1972; Karger Publishers; Volume: 145; Issue: 1 Linguagem: Inglês
10.1159/000252003
ISSN1421-9832
Autores Tópico(s)Skin Protection and Aging
ResumoA new, simple and quick method for the isolation of melanosomes from hair has been developed. The hairs were disintegrated by treatment with 1 n NaOH and the melanosomes separated by differential centrifugation. Electron microscopy examination confirmed that the results of this procedure are better than those obtained by any previously described method, both with regard to the purity and the intactness of the separated melanosomes. The methods of Gjesdal and of Bolt have been critically evaluated.
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