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Effect of oxygen abstraction on the peroxidase–luminol–perborate system: Relevance to the HRP enhanced chemiluminescence mechanism

1994; Wiley; Volume: 9; Issue: 4 Linguagem: Inglês

10.1002/bio.1170090405

1099-1271

Bibijana Cercek, Boris Cercek, Keith R. Roby, Lea Čerček,

Neutrophil, Myeloperoxidase and Oxidative Mechanisms

Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase-luminol-perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.