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Conversion of Urea or Ammonia into Essential Amino Acids (L-Leucine, L-Valine, and L-Isoleucine) Using Multienzyme Systems and Nadh-Dextran Immobilised in Artificial Cells

1987; Marcel Dekker; Volume: 15; Issue: 1 Linguagem: Inglês

10.3109/10731198709118530

0890-5533

Kang Fu Gu, Thomas Ming Swi Chang,

Enzyme Catalysis and Immobilization

Artificial cells containing leucine dehydrogenase (EC 1.4.1.9), alcohol dehydrogenase (EC 1.1.1.1; or glucose dehydrogenase, EC 1.1.1.47; or lactic dehydrogenase, EC 1.1.1.27; or malic dehydrogenase, EC 1.1.1.37), urease (EC 3.5.1.5) and dextran-NAD+ were prepared. Ammonia or urea could be converted into L-leucine, L-valine and L-isoleucine using artificial cells with four different multienzyme systems.