Portal de Periódicos da CAPES

The Comparative Growth Cycle of Different Strains of Endamoeba histolytica in Transparent Medium and of the Same Strain in Different Media

1950; American Society of Parasitologists; Volume: 36; Issue: 4 Linguagem: Inglês

10.2307/3273468

1937-2345

Ernest Carroll Faust, E. H. Sadun, Martha Grace Everritt, John L. Bradin, Ruth A. Lewis,

Parasitic infections in humans and animals

Many investigations have been undertaken in the attetnpt to provide a better understanding of the factors responsible for the growth of Endalmoeba histolytica in vitro. In order to evaluate the culturability of different strains of this ameba in the same medium, Faust and his co-workers (1946) cultured stools from 18 infected persons. Although no numerical determinations were carried out, these workers reported marked differences in population growth among the different strains. Since the bacterial flora present in these amebic cultures varied considerably, it was impossible to determine whether the differences in population growth were due to intrinsic properties of the amebae or to their accompanying bacterial flora. The cultivation of E. histolytica in a transparent medium without demonstrable bacterial growth has been reported by Shaffer and Frye (1948). This medium appears to be ideally suited for the study of factors influencing the growth of E. histolytica, since very little, if any, multiplication of bacteria appears to take place in it. The present study was carried out in an attempt to trace the growth of different strains of E. histolytica in this medium with an inhibited monobacterial flora and to compare it to that that in standard media with a multibacterial flora.