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STUDIES ON THE EXTRACELLULAR PROTEOLYTIC ENZYMES OF RHIZOPUS OLIGOSPORUS

1965; Canadian Science Publishing; Volume: 11; Issue: 4 Linguagem: Inglês

10.1139/m65-096

1480-3275

Hwa L. Wang, C. W. Hesseltine,

Algal biology and biofuel production

Two proteolytic enzyme systems were observed in the culture filtrates of Rhizopus oligosporus. One has an optimum pH at 3.0; the other, at 5.5. Both enzyme systems have maximum activities at 50–55 °C and are fairly stable at pH 3.0–6.0. Maximum production of the enzymes occurred after 72 to 96 hours of incubation and then it decreased rapidly. Higher proteolytic activity was noted in the culture filtrates of the organism grown in wheat flour medium than in soybean flour. Data suggest that formation of the enzyme systems appears to be inhibited by soybean extracts.